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Human Glypican 1 / GPC1 (S486A, S488A, S490A) Protein, His Tag, low endotoxin (MALS verified)

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GP1-H52H8-100ug
$390.00
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GP1-H52H8-1mg (500ug X 2)
$2,945.00
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GP1-H52H8
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Glypican 1, GPC1

Source

Human Glypican 1 (S486A, S488A, S490A), His Tag (GP1-H52H8) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1 (S486A, S488A, S490A)).

Predicted N-terminus: Asp 24

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 57.9 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.