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Product Details
Synonyms
FLT3LG, FL, FLT3L, Flt3 ligand
Source
Canine Flt-3 Ligand Protein, His Tag (FLD-D52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Arg 186 (Accession # A0A8C0M0A9).
Predicted N-terminus: Thr 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.6 kDa. The protein migrates as 29-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Canine Flt-3 Ligand Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Canine Flt-3 Ligand Protein, His Tag (Cat. No. FLD-D52H3) is more than 85% and the molecular weight of this protein is around 46-58 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Flt-3, Fc Tag (Cat. No. FL3-H5258) at 2 μg/mL (100 μL/well) can bind Canine Flt-3 Ligand Protein, His Tag (Cat. No. FLD-D52H3) with a linear range of 0.1-0.3 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Fms-related tyrosine kinase 3 ligand
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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