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Product Details
Synonyms
Fc gamma RIV, CD16-2, Fcgr4
Source
Biotinylated Mouse CD16-2 Protein, His,Avitag (FC4-M82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).
Predicted N-terminus: Gly 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Mouse CD16-2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) was greater than 85% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 0.186 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with an affinity constant of 31.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Biotinylated Mouse CD16-2 Protein, His,Avitag (Cat. No. FC4-M82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 0.394 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Customer Reviews Writing Reviews
J***y2023-06-24
LikesI ordered this biotinylated mouse Fc gamma RIV material to test the kinetics of Fc engineered mouse antibodies. The Fc receptor arrived quickly. The certificate of analysis was complete and provided useful recommendations for reconstituting the lyophilized material as well as suggested short and long-term storage conditions. I used the material in Biacore (SPR) using Biotin CAP chip. The material worked well in this assay format and we were able to draw useful conclusions.
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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