Biotinylated Mouse Fc gamma RIV / CD16-2 Protein, His,Avitag™ (HPLC & BLI verified)

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Menge
FC4-M82E8-25ug
$360.00
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FC4-M82E8-200ug
$1,565.00
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FC4-M82E8
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Synonyms

Fc gamma RIV, CD16-2, Fcgr4

Source

Biotinylated Mouse CD16-2 Protein, His,Avitag (FC4-M82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Gln 203 (Accession # A0A0B4J1G0-1).

Predicted N-terminus: Gly 21

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.

Background Introduction

FcgR4(Low affinity immunoglobulin gamma Fc region receptor IV) is also known as CD16-2, FcgammaRIV, receptor for the Fc region of immunoglobulin gamma . Also acts as a receptor for the Fc region of immunoglobulin epsilon . Binds with intermediate affinity to both IgG2a and IgG2b . Does not display binding to IgG1 or IgG3. Plays a role in promoting bone resorption by enhancing osteoclast differentiation following binding to IgG2a. Binds with low affinity to both the a and b allotypes of IgE. Has also been shown to bind to IgE allotype a only but not to allotype b. Binding to IgE promotes macrophage-mediated phagocytosis, antigen presentation to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions