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Product Details
Synonyms
ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
Source
Human ErbB3, Fc Tag (ER3-H5259) is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Thr 643 (Accession # NP_001973.2).
Predicted N-terminus: Ser 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 94.8 kDa. The protein migrates as 130-170 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human ErbB3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ErbB3, Fc Tag (Cat. No. ER3-H5259) is more than 95% and the molecular weight of this protein is around 205-255 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human NRG1 Beta 1 Protein, His Tag, premium grade (Cat. No. NR1-H5246) at 5 μg/mL (100 μL/well) can bind Human ErbB3, Fc Tag (Cat. No. ER3-H5259) with a linear range of 0.2-1 ng/mL (QC tested).
Protocol
Immobilized Human ErbB3, Fc Tag (Cat. No. ER3-H5259) at 2 μg/mL (100 μL/well) can bind Human NRG1-beta 1 Protein with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human NRG1 Beta 1 Protein, His Tag, premium grade (Cat. No. NR1-H5246) on HIS1K Biosensor, can bind Human ErbB3, Fc Tag (Cat. No. ER3-H5259) with an affinity constant of 1.32 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Receptor tyrosine-protein kinase erbB-3
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
31 Details
- Highest R&D Stage：
Approved
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