Human cytomegalovirus (strain Merlin) (HHV-5) Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (MALS verified)

Synonyms

Envelope protein H&L&UL128&UL130&UL131

Source

Human cytomegalovirus Envelope protein H&L&UL128&UL130&UL131, Twin-Strep Tag&His Tag (ENN-V5283) is expressed from human 293 cells (HEK293). It contains AA Ala 31 - Arg 278 & Arg 24 - Leu 719 (H&L) & Glu 28 - Gln 171 (UL128) & Ser 26 - Val 214 (UL130) & Gln 19 - Asn 129 (UL131) (Accession # Q6SW67 & F5HCH8 (H and L) & V9LLX6 (UL128) & F5HCP3 (UL130) & F5HET4 (UL131)).

Predicted N-terminus: Trp (H&L) & Glu 28 (UL128) & Ser 26 (UL130) & Gln 19 (UL131)

Molecular Characterization

This protein carries a twin strep tag and polyhistidine tag.

The protein has a calculated MW of 110.8 kDa (H&L) & 16.7 kDa (UL128) & 23.7 kDa (UL130) & 13.0 kDa (UL131). The protein migrates as 15 kDa, 35 kDa and 130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterologous pentamer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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