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E. coli Elongation Factor 4 Protein, His Tag (active enzyme)

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Product Number/Spec
Price
Menge
EF4-E5143-50ug
$450.00
EF4-E5143-500ug
$2600.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    Elongation Factor 4

  • Source

    E. coli Elongation Factor 4 Protein, His Tag (EF4-E5143) is expressed from E. coli cells. It contains AA Lys 2 - Lys 599 (Accession # C4ZYJ2).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Elongation Factor 4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 68.5 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5 M NaCl, 1 M Arginine, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    Elongation Factor 4 SDS-PAGE

    E. coli Elongation Factor 4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity

    The specific activity of Elongation Factor 4 was determined to be > 1700 pmol/min/mg in a GTPase-Glo assay using GTP solution substrate (QC tested).

    Protocol

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Publish Comment

Background Introduction

Elongation Factor 4 (EF4), also known as ​​LepA​​, is a highly conserved ​​GTPase translation factor​​ present across bacteria, mitochondria, and chloroplasts. It regulates protein synthesis by interacting with the ribosome during translation elongation. Unlike canonical elongation factors (e.g., EF-G) that promote forward translocation, EF4 catalyzes ​​back-translocation of tRNAs​​ on the ribosome, effectively acting as a molecular ’reverse gear‘ to reposition tRNAs and mRNA. This unique function allows EF4 to maintain translational fidelity under stress conditions (e.g., low pH or high ionic strength) by rescuing stalled ribosomes and correcting errors in protein synthesis.

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