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Product Details
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Biotinylated Human EGF R, Fc,Avitag (EGR-H82F8) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 96.7 kDa. The protein migrates as 120-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human EGF R, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human EGF R, Fc,Avitag (Cat. No. EGR-H82F8) is more than 90% and the molecular weight of this protein is around 202-246 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human EGF R, Fc,Avitag (Cat. No. EGR-H82F8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) (Cat. No. FRT-TA11) is used as the Acceptor. Human HGF R, His Tag (Cat. No. MET-H5227), at 10 ng/well and Biotinylated Human EGF R, Fc,Avitag (Cat. No. EGR-H82F8), at 10 ng/well, can bind to the Amivantamab with a linear detection range of 4-0.016 μg/mL (Routinely tested).
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Epidermal growth factor receptor erbB1
- Category：
- Listed Drugs Count：
39 Details
- Clinical Drugs Count：
206 Details
- Highest R&D Stage：
Approved
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