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Product Details
Synonyms
EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Source
Human EGF R Protein, Tag Free (EGR-H5213) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 68.7 kDa. The protein migrates as 95-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human EGF R Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human EGF R Protein, Tag Free (Cat. No. EGR-H5213) is more than 90% and the molecular weight of this protein is around 65-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human EGF R Protein, Tag Free (Cat. No. EGR-H5213) at 1 μg/mL (100 μL/well) can bind Human Anti-EGF R antibody with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Epidermal growth factor receptor erbB1
- Category：
- Listed Drugs Count：
39 Details
- Clinical Drugs Count：
206 Details
- Highest R&D Stage：
Approved
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