Save Product
Product Details
Synonyms
DSG-3
Source
Human DSG-3 Protein, His Tag (DS3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 50 - Arg 615 (Accession # P32926).
Predicted N-terminus: Glu 50
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 64.8 kDa. The protein migrates as 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.05 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human DSG-3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DSG-3 Protein, His Tag (Cat. No. DS3-H52H3) is more than 90% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DSG-3 Protein, His Tag (Cat. No. DS3-H52H3) at 1 μg/mL (100 μL/well) can bind DSG3 Recombinant Monoclonal Antibody-mIgG1 with a linear range of 0.02-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
DSG3 Recombinant Monoclonal Antibody-mIgG1 captured on CM5 chip via anti-mouse antibodies surface can bind Human DSG-3 Protein, His Tag (Cat. No. DS3-H52H3) with an affinity constant of 1.12 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Contact Us
Drug Candidate Licensing
