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Product Details
Synonyms
C2, Complement C2, Complement Component C2
Source
Human Complement C2, His Tag (CO2-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 752 (Accession # P06681-1).
Predicted N-terminus: Ala 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-105 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human Complement C2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Complement C2, His Tag (Cat. No. CO2-H52H7) is more than 95% and the molecular weight of this protein is around 90-115 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Complement C2, His Tag (Cat. No. CO2-H52H7) at 1 μg/mL (100 μL/well) can bind Empasiprubart with a linear range of 0.01-1 ng/mL (Routinely tested).
Protocol
Bioactivity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Complement C2
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
1 Details
- Highest R&D Stage：
Phase 3 Clinical
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