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Product Details
Synonyms
Coagulation factor VII, F7, SPCA, Proconvertin
Source
Human Coagulation factor VII, His Tag (TF7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 61 - Pro 466 (Accession # P08709-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.1 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Coagulation factor VII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Coagulation factor VII, His Tag (Cat. No. TF7-H52H5) is more than 85% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
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Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg, as measured under the described conditions (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Coagulation factor VII
- Category：
- Approved Drugs：
21 Details
- Drugs in Clinical Trials：
10 Details
- Highest Development Stage：
Approved
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