Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag (Nanodisc)

AIDD‑Ready
Related Products: Cell line CHEK-ATP139
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Menge
CL9-H5283-20ug
$1,440.00
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CL9-H5283-100ug
$5,355.00
CL9-H5283-500ug
$12,920.00
CL9-H5283
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Application
  • Cell based assay (FACS or functional assay)
  • Affinity testing (SPR / BLI)
  • Screening (ELISA)
Synonyms

CLDN9

Source

Nanodisc Human Claudin-9 / CLDN9 Full Length Protein, His,Twin-Strep Tag (CL9-H5283) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 217 (Accession # O95484-1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus, and a twin-strep tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.

Structure

Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

Synthesis Process

Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

Background Introduction

A tight junction protein with expression restricted to specific epithelia and the inner ear. It plays a role in paracellular ion permeability and auditory function. Its upregulation in certain cancers, including liver and pituitary tumors, marks it as an emerging target for antibody-based drug delivery.