Biotinylated Human Claudin-6 Full Length Protein, His,Avitag™&Flag Tag (Nanodisc)

AIDD‑Ready
Related Products: Cell line CHEK-ATP138, Streptavidin Series Products
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CL6-H85D4-20ug
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$5,870.00
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CL6-H85D4
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Application
  • Cell based assay (FACS or functional assay)
  • Affinity testing (SPR / BLI)
  • Screening (ELISA)
Synonyms

Claudin-6, CLDN6

Source

Nanodisc Biotinylated Human Claudin-6 Full Length Protein, His,Avitag™&Flag Tag (CL6-H85D4) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 220 (Accession # P56747).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus and a Flag tag at the C-terminus followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.6 kDa.

*The isotype control of empty/mock nanodisc (Cat. No. APO-H81Q5) is sold separately and not included in protein, you can follow the link for product information.

Structure

Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

Synthesis Process

Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>80% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

Background

An oncofetal tight junction protein absent in normal adult tissues but aberrantly expressed in ovarian, endometrial, and testicular cancers. Its strict cancer specificity and extracellular accessibility make it an ideal candidate for CAR-T cell therapy and bispecific antibody development.