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Product Details
Synonyms
AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS
Source
Human CFH Protein, His Tag (CFH-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 868 - Arg 1231 (Accession # P08603).
Predicted N-terminus: Ile 868
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 42.7 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CFH Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CFH Protein, His Tag (Cat. No. CFH-H52H3) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CFH Protein, His Tag (Cat. No. CFH-H52H3) at 10 μg/mL (100 μL/well) can bind Heparin Biotin with a linear range of 0.01-1.25 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H52W4) immobilized on CM5 Chip can bind Human CFH Protein, His Tag (Cat. No. CFH-H52H3) with an affinity constant of 4.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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