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Human CD72 Protein, His Tag (MALS verified)

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Product Number/Spec
Price
Menge
CD2-H52H3-100ug
$310.00
CD2-H52H3-1mg
$2245.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    CD72, Lyb-2, Lyb2

  • Source

    Human CD72, His Tag (CD2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # P21854-1).

    Predicted N-terminus: Arg 117

    Request for sequence
  • Molecular Characterization

    CD72 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.0 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    CD72 SDS-PAGE

    Human CD72, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD72 SEC-MALS

    The purity of Human CD72, His Tag (Cat. No. CD2-H52H3) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD72 ELISA

    Immobilized Human CD72, His Tag (Cat. No. CD2-H52H3) at 1 μg/mL, add increasing concentrations of Anti-Human CD72 Nanobody and then add Anti-Camelid VHH antibody at 1 μg/mL. Detection was performed using Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) with sensitivity of 0.15 μg/mL (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.

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