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Product Details
Synonyms
CD45, PTPRC, L-CA, T200
Source
Mouse CD45 protein, His Tag (CD5-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Lys 566 (Accession # P06800-4 ).
Predicted N-terminus: Gln 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.6 kDa. The protein migrates as 100-135 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse CD45 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse CD45 protein, His Tag (Cat. No. CD5-M52H3) is more than 90% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Mouse CD45 protein, His Tag (Cat. No. CD5-M52H3) captured on CM5 chip via anti-His antibody can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 7.19 µM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Leukocyte common antigen
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
7 Details
- Highest R&D Stage：
Phase 3 Clinical
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