Human CD40 Ligand / TNFSF5 Protein, Fc Tag (N297A), active trimer, premium grade

CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39

Human CD40 Ligand Protein, Fc Tag (N297A), premium grade (CDL-H5264) is expressed from human 293 cells (HEK293). It contains AA Gly 116 - Leu 261 (Accession # P29965-1).

Predicted N-terminus: Pro

It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Less than 0.01 EU per μg by the LAL method / rFC method.

<5 ppm of protein tested by ELISA.

<0.5 ng/µg of protein tested by ELISA.

<0.02 ng/μg of protein tested by qPCR.

Negative

Negative

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 24 months in lyophilized state;
• -70°C for 3 months under sterile conditions after reconstitution.