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Product Details
Synonyms
CD4, CD4mut, LEU3
Source
Rhesus macaque CD4, Fc Tag (CD4-C5256) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # G7N5T8).
Predicted N-terminus: Lys 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.0 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Rhesus macaque CD4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rhesus macaque CD4, Fc Tag (Cat. No. CD4-C5256) is more than 95% and the molecular weight of this protein is around 145-155 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rhesus macaque CD4, Fc Tag (Cat. No. CD4-C5256) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.4-25 ng/mL (QC tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 10 μg/mL (100 μL/well) can bind Rhesus macaque CD4, Fc Tag (Cat. No. CD4-C5256) with a linear range of 0.04-0.31 μg/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
T-cell surface antigen CD4
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
25 Details
- Highest R&D Stage：
Approved
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Drug Candidate Licensing
