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Product Details
Synonyms
CD39, ENTPD1, NTPDase 1, Entpd1, Ecto-ATPDase 1, Ecto-ATPase 1
Source
Biotinylated Human CD39 (R138A, M139A, E142K) Protein, His,Avitag (CD9-H82E8) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1 (R138A, M139A, E142K)).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 53.9 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human CD39 (R138A, M139A, E142K) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Biotinylated Human CD39 (R138A, M139A, E142K) Protein, His,Avitag (Cat. No. CD9-H82E8) at 1 μg/mL (100 μL/well)on streptavidin precoated (Cat. No. STN-N5116) (0.5 μg/well) plate can bind Anti-CD39 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Bioactivity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 8000 pmol/min/μg (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Ectonucleoside triphosphate diphosphohydrolase 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
11 Details
- Highest R&D Stage：
Phase 2 Clinical
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