Cynomolgus CD3 epsilon (22-48) Protein, Fc Tag, low endotoxin (MALS verified)

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CDE-C5253-100ug
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CDE-C5253-1mg
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CDE-C5253
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Synonyms

FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

Source

Cynomolgus CD3 epsilon (22-48) Protein, Fc Tag (CDE-C5253) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Thr 48 (Accession # Q95LI5 ).

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 29.3 kDa. The protein migrates as 33-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

The Gln22-Thr48 region of Cynomolgus CD3E is a core part of its extracellular immunoglobulin-like (Ig-like) domain.
Its primary function is to act as a crucial structural scaffold, mediating proper interactions with other CD3 subunits (specifically CD3G and CD3D).
It is also considered as SP34 binding site.