GENPower™ NLS-Cas9 Nuclease, premium grade

GENPower™ NLS-Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 protein purified from Escherichia coli for CRISPR-based genome editing. The introduction of nuclear localization signals (NLS) can help Cas9 enter the nucleus, increasing the chance of genomic DNA cleavage.

• High cleavage activity in different cell line
• High purity (＞95% SDS-PAGE / SEC-MALS)
• Animal-Free materials
• Batch-to-batch consistency
• Stringent quality control tests

10 mg/mL

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

≤10 ng/mg of protein tested by ELISA.

≤1 ng/mg of protein tested by qPCR.

Negative

Negative

Less than 0.01 EU per μg by the LAL method / rFC method.

Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH7.5 with protectants.

Contact us for customized product form or formulation.

For small-volume preparations, it is recommended to briefly centrifuge the vial prior to use to ensure that the solution is collected at the bottom of the vial. Use low-retention pipette tips to minimize sample loss during handling.

This product is supplied and shipped on dry ice.

• The product MUST be stored at -20°C or lower upon receipt;
• -20°C for 18 months under sterile conditions.