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Product Details
Synonyms
AGMX1, ATK, BPK
Source
Human BTK (1-659) Protein, His Tag (BTK-H55H4) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ser 659 (Accession # Q06187-1).
Predicted N-terminus: Met
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 78.2 kDa . The protein migrates as 57 kDa and 75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Data Display
SDS-PAGE
Human BTK (1-659) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human BTK (1-659) Protein, His Tag (Cat. No. BTK-H55H4) was greater than 85% as determined by SEC-HPLC.
Bioactivity
The BTK assay is performed using the ADP-GloTM Kinase Assay kit which quantifies the amount of ADP produced by the BTK reaction. The ADP- GloTM Reagent is added to terminate the kinase reaction and to deplete the remaining ATP, and then the Kinase Detection Reagent is added to convert ADP to ATP and to measure the newly synthesized ATP using luciferase/luciferin reaction.The specific activity is >20 pmol/min/ug (QC tested).
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