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Product Details
Synonyms
BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin-2, Beta-Secretase, β-secretase
Source
Human BACE-1, His Tag (BA1-H5220) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Thr 457 (Accession # NP_036236.1).
Predicted N-terminus: Thr 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.3 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human BACE-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human BACE-1, His Tag (Cat. No. BA1-H5220) is more than 95% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Report
Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >2.5 pmol/min/µg, as measured under the described conditions (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Beta-secretase 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
3 Details
- Highest R&D Stage：
Phase 3 Clinical
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