user
menu
letter
Sign InRegister
langEnglisharrow
1/8
+5 View all
COADS MSDS

Human B7-H3 (4Ig) / B7-H3b Protein, His Tag (Mass Spec & MALS verified)

AIDD‑Ready
Related Products: Cell line SCCHO-ATP169
1/8
+5 View all
COADS MSDS
Cat. No. / Size
Price
Availability
Qty
B7B-H52E7-100ug
$355.00
In Stock - Delivery in 1-2 business days
B7B-H52E7-1mg (500ug X 2)
$2,695.00
In Stock - Delivery in 1-2 business days
B7B-H52E7
Sub-Total :
$ 0
Customize Product Request Quote
Contact Us
Contact Us
Sub-Total:
$ 0
Request Quote
Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

4Ig-B7-H3, B7-H3, CD276, PSEC0249, UNQ309, PRO352, B7 homolog 3

Source

Human B7-H3 (4Ig), His Tag (B7B-H52E7) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 48.5 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Mass Spectrometry

  • Measured Molecular Mass

    The molecular weight by mass spectrometry is 48535 & 48408 Da (Routinely tested).

  • Sequence Coverage

    Human B7-H3 (4Ig), His Tag(B7B-H52E7) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 99.6% sequence coverage (Routinely tested).

  • N-terminal Sequence Analysis

    chain1: Gly-Ala-Leu-Glu-Val-Gln-Val-Pro-Glu-Asp-Pro-Val-Val-Ala-Leu chain2: Leu-Glu-Val-Gln-Val-Pro-Glu-Asp-Pro-Val-Val-Ala-Leu-Val-Gly
    (Routinely tested)

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.