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Product Details
Synonyms
APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2
Source
Mouse APRIL Protein, His Tag (APL-M5243) is expressed from human 293 cells (HEK293). It contains AA Ala 96 - Leu 241 (Accession # Q9D777).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 51.4 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse APRIL Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse APRIL Protein, His Tag (Cat. No. APL-M5243) is more than 95% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse BCMA, Fc Tag (Cat. No. BCA-M5258) at 2 μg/mL (100 μL/well) can bind Mouse APRIL Protein, His Tag (Cat. No. APL-M5243) with a linear range of 0.002-0.313 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse APRIL Protein, His Tag(Cat. No. APL-M5243) immobilized on CM5 Chip can bind Cynomolgus / Rhesus macaque BCMA, Fc Tag (Cat. No. BCA-C5253) with an affinity constant of 0.0488 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Mouse APRIL Protein, His Tag(Cat. No. APL-M5243) immobilized on CM5 Chip can bind Biotinylated Human TACI, Fc,Avitag (Cat. No. TAI-H82F6) with an affinity constant of 417 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 13
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
30 Details
- Highest Development Stage：
Approved
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