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Biotinylated Monomeric Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)

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Product Number/Spec
Price
Menge
AC2-H82E8-25ug
$450.00
AC2-H82E8-200ug
$1775.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    ACE-2, ACEH, ACE2

  • Source

    Biotinylated Monomeric Human ACE2, His,Avitag (AC2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 611 (Accession # Q9BYF1-1).

    Predicted N-terminus: Gln 18

    Request for sequence
  • Molecular Characterization

    ACE2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 72.3 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    ACE2 SDS-PAGE

    Biotinylated Monomeric Human ACE2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    ACE2 SEC-MALS

    The purity of Biotinylated Monomeric Human ACE2, His,Avitag (Cat. No. AC2-H82E8) is more than 90% and the molecular weight of this protein is around 75-90 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ACE2 ELISA

    Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E8) with a linear range of 0.039-0.625 μg/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Frontier Progress

 
Drug Development Progress
  • English Name:

    Angiotensin-converting enzyme 2

  • Category:

  • Listed Drugs Count:

    0 Details

  • Clinical Drugs Count:

    7 Details

  • Highest R&D Stage:

    Phase 2 Clinical

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