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Product Details
Features and Advantages
- Native Conformation: Native sequences, tag-free and natural function.
- Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
- Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
- Lowest Endotoxin level (<0.01 EU/ug).
- Safety Assurance: Sterile filtration through 0.2 μm membrane.
- AOF: Animal origin-free raw materials throughout the production process.
Synonyms
4-1BB Ligand, TNFSF9, CD137L
Source
Human 4-1BB Ligand (71-254) Protein, Fc Tag, Research Grade (41L-H5266) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1).
Predicted N-terminus: Pro
Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 87.5 kDa. The protein migrates as 95 kDa±5 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Data Display
SDS-PAGE
Human 4-1BB Ligand (71-254) Protein, Fc Tag, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human 4-1BB Ligand (71-254) Protein, Fc Tag, Research Grade (Cat. No. 41L-H5266) is more than 90% and the molecular weight of this protein is around 195-235 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human 4-1BB Protein, Mouse IgG2a Fc Tag (Cat. No. 41B-H5256) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254) Protein, Fc Tag, Research Grade (Cat. No. 41L-H5266) with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Human 4-1BB Ligand (71-254) Protein, Fc Tag, Research Grade (Cat. No. 41L-H5266) induce IL‐8 secretion in HT1080 human CD137 cell line. The specific activity of Human 4-1BB Ligand (71-254) Protein, Fc Tag, Research Grade is > 1.00 x 10^5 U/mg (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 9
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 2 Clinical
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