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Product Details
Source
Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (CDE-CH64G0) is recombinantly produced from CHO cells.
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Isotype
Human IgG4, Kappa
Specificity
This product is a specific antibody specifically reacts with CD3 epsilon.
Endotoxin
Less than 0.002 EU per μg by the LAL method / rFC method.
Protein A
<5 ppm of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Sterility
Negative
Mycoplasma
Negative
Formulation
Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
For long term storage, the product should be stored at liquid state at -70°C.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 2-8°C for 12 months under sterile conditions;
- -70°C for 24 months.
ACRO Quality Management System
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Data Display
SDS-PAGE
Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (Cat. No. CDE-CH64G0) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
Bioactivity-CELL BASE
Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (Cat. No. CDE-CH64G0) stimulates secretion of IL-2 by PBMC stimulated with Monoclonal Anti-Human CD28 Antibody, Mouse IgG1. The typical EC50 for this effect is 0.1442 ng/mL (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (Cat. No. CDE-CH64G0) with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
User Reviews Publish Comment
FAQ
- Product
Does OKT3 bind to CD3E monomers or CD3E-containing heterodimers?
OKT3 binds to the CD3E subunit within CD3E&CD3D and CD3E&CD3G heterodimers, but it does not bind to monomeric CD3E.
Background Introduction
Immunoglobulin 4 (IgG4) is one of four human IgG subclasses and has several unique functional characteristics. It exhibits low affinity for complement and for most Fc receptors. It furthermore has generally high affinity for its antigen, with binding occurring in a monovalent fashion, as IgG4 can exchange Fab-arms with other IgG4 molecules. Because of these characteristics, IgG4 is believed to block its targets and prevent inflammation, which, depending on the setting, can have a protective or pathogenic effect.
Frontier Progress
Drug Development Progress- English Name：
T cell surface glycoprotein CD3
- Category：
- Listed Drugs Count：
10 Details
- Clinical Drugs Count：
198 Details
- Highest R&D Stage：
Approved
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