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TPBG, 5T4, M6P1, 5T4AG, WAIF1, 5T4 oncofetal antigen, Trophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein
Unconjugated Human TPBG, His,Avitag (TPG-H52E5) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 355 (Accession # Q13641-1).
Predicted N-terminus: Ser 32
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
TPG-H52E5 is the unconjugated version of TPG-H82Eb (Biotinylated Human TPBG, His,Avitag). It can be utilized for assay development or customized biotinylation according to the experimental designs.
Less than 0.01 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.