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Product Details
Synonyms
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
SARS-CoV-2 S protein (K417N,D614G), His Tag (SPN-C52Hm) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (K417N, D614G) were identified in the SARS-CoV-2 variant (known as B.1.351 or 20C/501Y.V2) which emerged in South Africa. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Val 16
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
SARS-CoV-2 Spike Trimer (K417N, D614G), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 Spike Trimer (K417N, D614G), His Tag (Cat. No. SPN-C52Hm) is more than 85% and the molecular weight of this protein is around 680-720 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer , His Tag (Cat. No. SPN-C52Hm) with a linear range of 1.6-50 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike Trimer , His Tag (Cat. No. SPN-C52Hm) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 3.1-50 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Coronavirus spike glycoprotein
- Category：
- Listed Drugs Count：
15 Details
- Clinical Drugs Count：
88 Details
- Highest R&D Stage：
Approved
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