Human SLC1A5 Protein, His Tag, TrxA Tag

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SL5-H5149-100ug
$360.00
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SL5-H5149-1mg (100ug X 10)
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SL5-H5149
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Synonyms

ASCT2, M7V1, RDR, RDRC, ATB(0), AAAT, ASCT2M7VS1, M7V1ATBO, R16, RD114, RDRCFLJ31068

Source

Human SLC1A5, His Tag, TrxA Tag (SL5-H5149) is expressed from E. coli cells. It contains AA Gln 154 - Gln 224 (Accession # Q15758-1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus, followed by a TrxA tag. The protein has a calculated MW of 22.5 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background Introduction

Sodium-dependent amino acids transporter(SLC1A5) that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904).