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Rabbit PD-L1 / B7-H1 Protein, His Tag (MALS verified)

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Product Number/Spec
Price
Menge
PDL-R52H6-100ug
$360.00
PDL-R52H6-1mg
$2615.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

  • Source

    Rabbit PD-L1, His Tag (PDL-R52H6) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - His 238 (Accession # G1SUI3-1).

    Predicted N-terminus: Phe 19

    Request for sequence
  • Molecular Characterization

    PD-L1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.1 kDa. The protein migrates as 32-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    PD-L1 SDS-PAGE

    Rabbit PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    PD-L1 SEC-MALS

    The purity of Rabbit PD-L1, His Tag (Cat. No. PDL-R52H6) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     PD-L1 SPR

    Rabbit PD-L1, His Tag (Cat. No. PDL-R52H6) immobilized on CM5 Chip can bind Rabbit PD-1, His Tag (Cat. No. PD1-R52H0) with an affinity constant of 3.87 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Frontier Progress

 
Drug Development Progress
  • English Name:

    Programmed death-ligand 1

  • Category:

  • Listed Drugs Count:

    12 Details

  • Clinical Drugs Count:

    176 Details

  • Highest R&D Stage:

    Approved

Drug Candidate Licensing
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