SARS-CoV-2 Nucleocapsid protein (D3L, R203K, G204R, S235F), His Tag

Synonyms

Nucleocapsid protein, NP, Protein N

Source

SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C52H8) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). D3L/ R203K/ G204R/ S235F were identified in the SARS-CoV-2 Alpha variant (Pango lineage: B.1.1.7; other names: 20I/501Y.V1 or VOC 202012/01) (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).

Predicted N-terminus: Met 1

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.4 kDa. The protein migrates as 30-35 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process