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Product Details
Synonyms
NECTIN4, LNIR, PRR4, PVRL4
Source
Mouse Nectin-4, Fc Tag (NE4-M5257) is expressed from human 293 cells (HEK293). It contains AA Gly 31 - Ser 347 (Accession # Q8R007-1).
Predicted N-terminus: Gly 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 60.8 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse Nectin-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) is more than 85% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Nectin-1, His Tag (Cat. No. PV1-H5223) at 5 μg/mL (100 μL/well) on an Nickel Coated plate can bind Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) with a linear range of 0.156-2.5 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Mouse Nectin-4, Fc Tag (Cat. No. NE4-M5257) on Protein A Biosensor, can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with an affinity constant of 278 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Nectin-4
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
28 Details
- Highest R&D Stage：
Approved
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