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Product Details
Synonyms
NECTIN4, LNIR, PRR4, PVRL4
Source
Cynomolgus Nectin-4, His Tag (NE4-C52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # XP_005541277.1).
Predicted N-terminus: Gly 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.0 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus Nectin-4, His Tag (Cat. No. NE4-C52H4) is more than 85% and the molecular weight of this protein is around 35-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus Nectin-4, His Tag (Cat. No. NE4-C52H4) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Nectin-4 Antibody with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse Anti-Nectin-4 Antibody (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface can bind Cynomolgus Nectin-4, His Tag (Cat. No. NE4-C52H4) with an affinity constant of 824 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Cynomolgus Nectin-4, His Tag (Cat. No. NE4-C52H4) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Nectin-4
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
28 Details
- Highest R&D Stage：
Approved
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