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Human LSECtin / CLEC4G Protein, Fc Tag

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Product Number/Spec
Price
Menge
CLG-H5250-50ug
$300.00
CLG-H5250-1mg (250ug X 4)
$2040.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    CLEC4G, LSECtin

  • Source

    Human LSECtin, Fc Tag (CLG-H5250) is expressed from human 293 cells (HEK293). It contains AA Lys 55 - Cys 293 (Accession # Q6UXB4-1).

    Predicted N-terminus: Glu

    Request for sequence
  • Molecular Characterization

    LSECtin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 52.1 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    LSECtin SDS-PAGE

    Human LSECtin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85%.

  • Bioactivity-ELISA

     LSECtin ELISA

    Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 5 μg/mL (100 μL/well) can bind Human LSECtin, Fc Tag (Cat. No. CLG-H5250) with a linear range of 0.001-0.313 μg/mL (Routinely tested).

    Protocol
  •  LSECtin ELISA

    Immobilized Human LSECtin, Fc Tag (Cat. No. CLG-H5250) at 5 μg/mL (100 μL/well) can bind Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) with a linear range of 0.001-0.156 ng/mL (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

C-type lectin superfamily 4, member G (CLEC4G) is also known as liver and lymph node sinusoidal endothelial cell C-type lectin (LSECtin), which is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily. CLEC4G / LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner. In addition, CLEC4G / LSECtin has the ability to bind to surface glycoproteins of enveloped viruses. In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin-mediated infection of cells by Ebola virus has been demonstrated.

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