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Product Details
Product Details
Laminin 523 (LN523) is a key recombinant basement membrane protein that provides an ideal surface for the adhesion and growth of human pluripotent stem cells while significantly promoting their directed differentiation. Particularly in ophthalmology, it effectively guides the differentiation of stem cells into retinal progenitor cells and supports the formation of retinal organoids containing photoreceptor precursors, while also facilitating the generation and maturation of corneal epithelial cells. It serves as an essential substrate for studying visual development and disease mechanisms.
Expression System
HEK293
Key Parameter
Purity (SDS PAGE)> 90%Mycoplasma TestNegativeSterility TestNegativeEndotoxin Test<0.01 EU per μgHost Cell Protein<0.5 ng/µgHost Cell DNA<0.02 ng/μgFormulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
Bioactivity-SPR
Human Laminin 523 Protein, premium grade (Cat. No. LA3-H5217) immobilized on CM5 Chip can bind Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein, His Tag&Tag Free with an affinity constant of 98.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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FAQ
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Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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