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Human Kremen-2 CUB Domain Protein, His Tag

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Product Number/Spec
Price
Menge
KR2-H52H4-100ug
$545.00
KR2-H52H4-1mg (500ug X 2)
$3575.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    KREMEN2, Kremen-2, KRM2, Dickkopf receptor 2, Kringle domain-containing transmembrane protein 2

  • Source

    Human Kremen-2 CUB Domain Protein, His Tag (KR2-H52H4) is expressed from human 293 cells (HEK293). It contains AA Cys 219 - Leu 326 (Accession # Q8NCW0-1).

    Predicted N-terminus: Cys 219

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    KREMEN2 SDS-PAGE

    Human Kremen-2 CUB Domain Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     KREMEN2 ELISA

    Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) at 5 μg/mL (100 μL/well) can bind Human Kremen-2 CUB Domain Protein, His Tag (Cat. No. KR2-H52H4) with a linear range of 0.039-2.5 μg/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Kremen2 (Krm2) plays an important role in embryonic development, bone formation, and tumorigenesis as a crucial regulator of classical Wnt/β-catenin signaling pathway. Krm1 and its paralog Krm2 share the ability to bind Dkk1 and inhibit Wnt signaling, both processes previously shown to rely on the extracellular domain. Previous studies have shown that Krm2 may be involved in embryonic development, bone formation, neural ridge formation and tumorigenesis and could be a biomarker of grading and a potential therapeutic target in gastric cancer.

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