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Product Details
Synonyms
KREMEN, KRM1，Dickkopf receptor, Kringle domain-containing transmembrane protein 1, Kringle-containing protein marking the eye and the nose
Source
Human Kremen-1, Fc Tag (KR1-H5253) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Thr 392 (Accession # Q96MU8-1).
Predicted N-terminus: Arg 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Kremen-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 2 μg/mL (100 μL/well) can bind Human Kremen-1, Fc Tag (Cat. No. KR1-H5253) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human KREMEN1, Fc Tag (Cat. No. KR1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 283 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human KREMEN1, Fc Tag (Cat. No. KR1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein with an affinity constant of 114 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human KREMEN1, Fc Tag (Cat. No. KR1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) with an affinity constant of 241 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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