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Product Details
Synonyms
Integrin alpha V beta 6, ITGAV&ITGB6
Source
Mouse ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (IT6-M52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 988 (ITGAV) & Gly 22 - Pro 708 (ITGB6) (Accession # P43406-1 (ITGAV) & Q9Z0T9-1 (ITGB6)).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Mouse Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 112.5 kDa (ITGAV) and 80.2 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The non-reducing (NR) protein migrates as 135-145 kDa (ITGAV) & 80-90 kDa (ITGB6) respectively due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-M52W9) is more than 95% and the molecular weight of this protein is around 189-231 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-M52W9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) on SA Biosensor, can bind Mouse ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-M52W9) with an affinity constant of 8.28 nM as determined in BLI assay (ForteBio Octet R8)(Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Integrin alpha-V/beta-6
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
6 Details
- Highest R&D Stage：
Phase 2 Clinical
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