Rat Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Synonyms

Integrin alpha V beta 3, ITGAV&ITGB3

Source

Rat ITGAV&ITGB3 Heterodimer, His Tag&Tag Free (IT3-R52E2) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 988 (ITGAV) & Ser 27 - Asp 717 (ITGB3) (Accession # NP_001385621.1 (ITGAV) & Q8R2H2 (ITGB3)).

Predicted N-terminus: Phe 31 | Ser 27

Molecular Characterization

Rat ITGAV&ITGB3 Heterodimer, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 112.3 kDa (ITGAV) & 81.6 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 70-85 kDa (ITGB3) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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