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Product Details
Synonyms
IL21R, CD360, NILR
Source
Cynomolgus IL-21 R, Fc Tag (ILR-C5259) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Glu 232 (Accession # XP_005591578.2).
Predicted N-terminus: Cys 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.0 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus IL-21 R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Cynomolgus IL-21 R, Fc Tag (Cat. No. ILR-C5259) is more than 85% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus IL-21, His Tag (Cat. No. IL1-C52H9) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-21 R, Fc Tag (Cat. No. ILR-C5259) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cynomolgus IL-21 Protein, Fc Tag (Cat. No. IL1-C5253) immobilized on CM5 Chip can bind Cynomolgus IL-21 R, Fc Tag (Cat. No. ILR-C5259) with an affinity constant of 0.354 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin 21 Receptor
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 1 Clinical
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