Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)

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ILG-C5283-100ug
$350.00
ILG-C5283-1mg (500ug × 2)
$2,310.00
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分子别名(Synonym)

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统(Source)

Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer, His Tag&Twin-Strep Tag (ILG-C5283) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Thr 240 & Leu 23 - Pro 255 (Accession # Q38J85-1 & Q38JL2-1).

Predicted N-terminus: Ala 27 & Leu 23

Request for sequence

蛋白结构(Molecular Characterization)

IL-2 R beta & IL-2 R gamma Structure

Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 30.1 kDa (IL-2RB) and 34.1 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 35-44 kDa and 55-60 kDa respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

Background

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.