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Product Details
Synonyms
IL-2 R beta & IL-2 R alpha & IL-2 R gamma
Source
Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag (ILG-R5223) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 239 (IL-2RB) & Glu 22 - Gln 235 (IL-2RA) & Ser 25 - Ala 262 (IL-2RG) (Accession # P26896-1 (IL-2RB) & P26897-1 (IL-2RA) & Q68FU6-1 (IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Glu 22 (IL-2RA)
Request for sequenceMolecular Characterization
Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 30.2 kDa (IL-2RB) and 59.3 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 45 kDa and 110 kDa respectively due to glycosylation.
The protein is designed as a heterotrimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag (Cat. No. ILG-R5223) is more than 85% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag (Cat. No. ILG-R5223) with a linear range of 39-156 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Rat IL-2RB&IL-2RA&IL-2RG, His Tag&Twin-Strep Tag (Cat. No. ILG-R5223) on NTA Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 1.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-2
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
43 Details
- Highest R&D Stage：
Approved
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