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Product Details
Synonyms
IFNAR2, IFNARB, IFNABR, IFN-R-2, IFN-alpha, beta receptor 2
Source
Human IFNAR2, His Tag (IF2-H5224) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Lys 243 (Accession # P48551-2).
Predicted N-terminus: Ile 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.8 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human IFNAR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IFNAR2, His Tag (Cat. No. IF2-H5224) is more than 85% and the molecular weight of this protein is around 32-42 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Human IFN-alpha 2a (R46K) Protein, Fc Tag (Cat. No. IFA-H5253) with a linear range of 1-40 ng/mL (Routinely tested).
Protocol
Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Human IFN-beta Protein, Fc Tag (Cat. No. IFB-H5254) with a linear range of 0.03-2 ng/mL (Routinely tested).
Protocol
Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IFN-alpha 2b Protein, His,Avitag (Cat. No. IFB-H82E3) with a linear range of 0.002-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human IFN-alpha 2b Protein, Fc Tag (Cat. No. IFB-H5253) immobilized on CM5 Chip can bind Human IFNAR2, His Tag (Cat. No. IF2-H5224) with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IFNAR2, His Tag (Cat. No. IF2-H5224) on HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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