表达区间及表达系统（Source）

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein (HL1-H82W8) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01) (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01)).

Predicted N-terminus: Ile 26 & Gly 30

Request for sequence

蛋白结构（Molecular Characterization）

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01 Monomer Protein, produced by co-expression of HLA-DRA1*01:01 and HLA-DRB1*04:01, has a calculated MW of 29.2 kDa (HLA-DRA1*01:01) and 27.1 kDa (HLA-DRB1*04:01). Subunit HLA-DRA1*01:01 is fused with a polyhistidine tag at the C-terminus and subunit HLA-DRB1*04:01 is fused with an Avi tag (Avitag™) at the C-terminus. The protein migrates as 38-40 kDa and 31-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.