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Product Details
Synonyms
HLA-A*2402 & B2M
Source
PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (HLM-HP2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) ).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequenceMolecular Characterization
PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein is assembled by biotinylated monomer (HLM-H82W4) and PE-labeled streptavidin.
Biotinylated Human HLA-A*24:02&B2M Monomer Protein is produced by co-expression of HLA and B2M. This Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (Cat. No. HLM-HP2H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.3-2 ng/mL (QC tested).
Protocol
Immobilized PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (Cat. No. HLM-HP2H4) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.3-2 ng/mL (QC tested).
Protocol
Evaluation of CAR expression
FACS Analysis of Non-specific binding to PBMCs
Non-specificity of PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (Cat. No. HLM-HP2H4) binding to CD4+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with Alexa Fluor® 700 anti-human CD4 Antibody and PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (1 µg corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both Alexa 700 and PE positive signals was used to evaluate the non-specific binding activity to human CD4+ cells (Routinely tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
Non-specificity of PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (Cat. No. HLM-HP2H4) binding to CD8+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC anti-human CD8 Antibody and PE-Labeled Human HLA-A*24:02&B2M Tetramer Protein (1 µg corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD8+ cells (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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