Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified)

分子别名（Synonym）

HLA-A*0301 & B2M & KRAS (VVVGAGGVGK)

表达区间及表达系统（Source）

Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (HLS-H52H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGAGGVGK).

Predicted N-terminus: Gly 25 & Ile 21

Request for sequence

蛋白结构（Molecular Characterization）

Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide.

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 34.2 kDa and 11.7 kDa. The protein migrates as 39-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.