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APC-Labeled Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Tetramer Protein

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HLT-HA2H7-25ug
$410.00
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HLT-HA2H7-200ug (50ug X 4)
$2350.00
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Product Details

  • Synonyms

    HLA-A*0201 & B2M & hTERT (ILAKFLHWL)

  • Source

    APC-Labeled Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Tetramer Protein (HLT-HA2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ILAKFLHWL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ILAKFLHWL).

    Predicted N-terminus: Gly 25 & Ile 21

  • Molecular Characterization

    APC-Labeled Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Complex Protein is produced by co-expression of HLA and B2M loaded with hTERT peptide. Biotinylated Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background Introduction

hTERT (human Telomerase Reverse Transcriptase), the catalytic subunit of telomerase, maintains telomere length to drive tumor cells’ unlimited proliferation. It is highly expressed in ~85-90% of cancers but silenced in most normal somatic cells. The peptide ILAKFLHWL (from hTERT residues 540-548) acts as a tumor-specific antigen. It binds to MHC class I molecule HLA-A0201 to form a surface complex on tumors, activating cytotoxic T lymphocytes (CTLs). The Human HLA-A*0201 hTERT (ILAKFLHWL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and ILAKFLHWL peptide of the hTERT.

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