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APC-Labeled Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Tetramer Protein

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HLT-HA2H7-25ug
$410.00
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HLT-HA2H7-200ug (50ug X 4)
$2,350.00
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HLT-HA2H7
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Product Details
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Synonyms

HLA-A*0201 & B2M & hTERT (ILAKFLHWL)

Source

APC-Labeled Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Tetramer Protein (HLT-HA2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ILAKFLHWL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ILAKFLHWL).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

APC-Labeled Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.

Biotinylated Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Complex Protein is produced by co-expression of HLA and B2M loaded with hTERT peptide. Biotinylated Human HLA-A*02:01&B2M&hTERT (ILAKFLHWL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

Conjugate

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

hTERT (human Telomerase Reverse Transcriptase), the catalytic subunit of telomerase, maintains telomere length to drive tumor cells’ unlimited proliferation. It is highly expressed in ~85-90% of cancers but silenced in most normal somatic cells. The peptide ILAKFLHWL (from hTERT residues 540-548) acts as a tumor-specific antigen. It binds to MHC class I molecule HLA-A0201 to form a surface complex on tumors, activating cytotoxic T lymphocytes (CTLs). The Human HLA-A*0201 hTERT (ILAKFLHWL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and ILAKFLHWL peptide of the hTERT.