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Product Details
Synonyms
ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1
Source
Human Her2 (S310Y), His Tag (HE2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1 (S310Y)).
Predicted N-terminus: Thr 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 71.3 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. This protein has been shown to weakly bind to Pertuzumab in our functional ELISA studies.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human Her2 (S310Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Her2 (S310Y), His Tag (Cat. No. HE2-H52H3) is more than 85% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Her2 (S310Y), His Tag (Cat. No. HE2-H52H3) at 1 μg/mL (100 μL/well) can bind Trastuzumab Biosimilar with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Her2 (S310Y), His Tag (Cat. No. HE2-H52H3) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.2-12.5 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2 (S310Y), His Tag (Cat. No. HE2-H52H3) at 1 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.1-3 ng/mL, This protein has been shown to weakly bind to Pertuzumab in our functional ELISA studies.(Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Receptor protein-tyrosine kinase erbB-2
- Category：
- Listed Drugs Count：
47 Details
- Clinical Drugs Count：
238 Details
- Highest R&D Stage：
Approved
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